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品牌名称：

型号规格：

批准文号： 仅供科研使用

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销售渠道：医院

025-86978335

产品介绍：

【产品名称】

通用名称：大/小鼠瘦素检测试剂盒（酶联免疫法）

英文名称：Mouse-/Rat-Leptin ELISA

【预期用途】

用于小鼠和大鼠血清和血浆中瘦素的测定。仅供科研使用。不用于临床诊断。

【包装规格】

96T/盒

【背景知识】

瘦素是ob基因(1,2)的产物，是最近发现的一种单链蛋白激素，分子量为16 kD，被认为在体重调节中起关键作用。它的氨基酸序列与其他蛋白质没有主要同源性 (1)。瘦素几乎完全由分化的脂肪细胞产生 (3-5)。它作用于中枢神经系统，特别是下丘脑，抑制食物摄入并增加能量消耗 ( 2, 6-9). 瘦素受体有不同的剪接形式，它们的长度不同。它们属于细胞因子 1 类受体家族 (10-12)，在体内无处不在 (10,11,13,14)。许多瘦素结合蛋白之一代表瘦素受体的循环形式 (15)。除了对新陈代谢的影响外，瘦素还对广泛的内分泌轴有强大的影响。在雄性小鼠中，它分别减弱了饥饿引起的 LH、睾酮和甲状腺素的显着下降以及 ACTH 和皮质酮的增加。在雌性小鼠中，瘦素可防止饥饿引起的排卵延迟 (16)。由于 ob 突变而缺乏瘦素的 Oblob 小鼠是不育的。这种缺陷可以通过瘦素的应用来纠正，但不能通过禁食导致的体重减轻来纠正 (17)。这表明瘦素在生殖中起着重要作用。

瘦素的作用可以至少部分解释为瘦素对弓状核神经元神经肽 Y (NPY) 的形成和分泌的抑制作用 (6,18,19)。 NPY 是一种有效的食欲刺激剂 (20,21)，并且已知参与各种垂体激素的调节，例如通过刺激生长抑素抑制生长激素 (22,23)、抑制促性腺激素 (23) 和刺激垂体-肾上腺轴 (21)。

决定循环瘦素浓度的最重要变量是体脂肪量 (24-26)。在定期进食的情况下，瘦素反映了脂肪组织的比例 (27)。瘦素浓度随脂肪量呈指数增加 (37)。瘦素的组成型合成受多种非激素和激素因子的调节。啮齿动物和人类的刺激物是暴饮暴食 (28,29)、高脂肪饮食 (41)、胰岛素 (3,5,30-33) 和糖皮质激素 (5,34-36)。一般来说，瘦素是各种代谢和内分泌反馈回路的组成部分 (38)。

参考文献：

1. Zhang Y,Proenca R,Maffei M, Barone M, Leopold L， Friedman JM.1994 Positionalcloning of the mouse obese gene and its human homologue. Nature.372:425-432.

1. Halaas JL,Gajiwala KS,Maffei M, et al.1995 Weight-reducing effects of the plasmaprotein encoded by the obese gene.Science. 269:543-546.

2. MacDougald OA, Hwang CS, Fan H, Lane MD.1995 Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes.Proc Natl Acad ScisA.92:9034-9037.

3. Rentsch J , Chiesi M. 1996 Regulation of ob gene mRNA levels in cultured adipocytes.FEBS Lett. 379:55-59.

4. Wabitsch M,Jensen PB,Blum WF， et al. 1996 Insulin and cortisol promote leptinproduction in cultured human fat cells. Diabetes. 45:1435-1438.

5. Stephens TW,Basinski M,Bristow PK, et al.1995 The role of neuropeptide Y in theantiobesity action of the obese gene product. Nature.377:530-532.

6. Campfield LA, Smith FJ,Guisez Y,Devos R, Burn P.1995 Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neural networks.Science. 269:546-549.

7. Pelleymounter MA, Cullen MJ,Baker MB, et al. 1995 Effects of the obese gene producton body weight regulation in oblob mice. Science. 269:540-543.

8. Levin N, Nelson C, Gurney A, Vandlen R, de-Sauvage F.1996 Decreased food intakedoes not completely account for adiposity reduction after ob protein infusion.Proc Natl

Acad Sci u sA.93:1726-1730.

9. Tartaglia LA, Dembski M, Weng X, et al.1995 ldentification and expression cloning of a leptin receptor, OB-R.Cell.83:1263-1271.

10. Chen H, Charlat O, Tartaglia LA, et al.1996 Evidence that the diabetes gene encodesthe leptin receptor: identification of a mutation in the leptin receptor gene in dbldb mice.

Cell.84:491-495.

11.Lee GH, Proenca R,Montez JM, et al.1996 Abnormal splicing of the leptin receptor indiabetic mice. Nature.379:632-635.

12. Lynn RB, Cao GY,Considine RV, Hyde TM,Caro JF.1996 Autoradiographic localization of leptin binding in the choroid plexus of oblob and dbldb mice.Biochem Biophys ResCommun. 219:884-889.

13. Considine RV,Considine EL, Williams CJ, Hyde TM,Caro JF. 1996 The hypothalamic leptin receptor in humans: identification of incidental sequence polymorphisms andabsence of the dbl/db mouse and fa/fa rat mutations. Diabetes. 45:992-994.

14. Sinha MK, Opentanova l, Ohannesian JP, et al.1996 Evidence of free and bound leptinin human circulation.J Clin Invest.98:1277-1282.

15. Ahima RS,Prabakaran D,Mantzoros C, et al. 1996 Role of leptin in the neuroendocrineresponse to fasting. Nature.382:250-252.

16. Chehab FF, Lim ME, Lu R.1996 Correction of the sterility defect in homozygous obesefemale mice by treatment with the human recombinant leptin.Nat Genet. 12:318-320.17) Schwartz MW，Baskin DG,Bukowski TR, et al. 1996 Specificity of leptin action on elevated blood glucose levels and hypothalamic neuropeptide Y gene expression in oblobmice. Diabetes.45:531-535.

18. Schwartz MW,Seeley RJ,Campfield LA,Burn P,Baskin DG. 1996 ldentification oftargets of leptin action in rat hypothalamus.J Clin Invest.98:1101-1106.

19. Campfield LA, Smith FJ,Burn P.1996 The OB protein (leptin) pathway - a link betweenadipose tissue mass and central neural networks.Horm metab Res.28:619-632.

20. Rohner-Jeanrenaud F, Cusin l, Sainsbury A, Zakrzewska KE,Jeanrenaud B.1996 The loop system between neuropeptide Y and leptin in normal and obese rodents. Hormmetab Res. 28:642-648.

21. Chan YY,Steiner RA,Clifton DK. 1996 Regulation of hypothalamic neuropeptide-Yneurons by growth hormone in the rat. Endocrinol.137:1319-1325.

22. Pierroz DD, Catzeflis C,Aebi AC,Rivier JE,Aubert ML.1996 Chronic administration of neuropeptide Y into the lateral ventricle inhibits both the pituitary-testicular axis andgrowth hormone and insulin-like growth factor l secretion in intact adult male rats.Endocrinol.137:3-12.

23. Frederich RC，Hamann A, Anderson S, Lollmann B, Lowell BB,Flier JS.1995 Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin

action. Nat Med.1:1311-1314.

24. Maffei M,Halaas J，Ravussin E, et al. 1995 Leptin levels in human and rodent:measurement of plasma leptin and ob RNA in obese and weight-reduced subjects.NatMed.1:1155-1161.

25. Considine RV，Sinha MK,Heiman ML, et al. 1996 Serum immunoreactive-leptinconcentrations in normal-weight and obese humans.N Engl J Med.334:292-295.

26. Kolaczynski Jw，Considine RV,Ohannesian J, et al. 1996 Responses of leptin toshort-term fasting and refeeding in humans: A link with ketogenesis but not ketones themselves. Diabetes. 45:1511-1515.

27. Harris RB,Ramsay TG, Smith SR, Bruch RC.1996 Early and late stimulation of ob mRNAexpression in meal-fed and overfed rats.J Clin Invest.97:2020-2026.

28. Kolaczynski Jw,Ohannesian J,Considine RV,Marco C, Caro JF.1996 Response of leptin to short-term and prolonged overfeeding in humans.J Clin Endocrinol metab.

91:4162-4165.

29. Saladin R,De-Vos P,Guerre-Millo M, et al. 1995 Transient increase in obese geneexpression after food intake or insulin administration. Nature.377:527-529.

30. Cusin I, Sainsbury A, Doyle P, Rohner-Jeanrenaud F, Jeanrenaud B.1995 The ob gene and insulin. A relationship leading to clues to the understanding of obesity.Diabetes.44:1467-1470.

31. Kolaczynski JW,Nyce MR,Considine RV, et al.1996 Acute and chronic effects of insulinon leptin production in humans: studies in vivo and in vitro. Diabetes.45:699-701.

32. Malstrom R, Taskinen M-R, Karonen S-L，Yki-Jarvinen H.1996 Insulin increases plasma leptin concentrations in normal subjects and patients with NIDDM. Diabetologia.39:993-996.

33. De-Vos P, Saladin R，Auwerx J,Staels B.1995 Induction of ob gene expression by corticosteroids is accompanied by body weight loss and reduced food intake.J BiolChem. 270:15958-15961.

34. Slieker LJ,Sloop KW,Surface PL, et al.1996 Regulation of expression of ob mRNA and protein by glucocorticoids and cAMP.J Biol Chem. 271:5301-5304.

35. Miell JP, Englaro P, Blum WF.1996 Dexamethasone induces an acute and sustainedrise in circulating leptin levels in normal human subjects. Horm metab Res.28:704-707.36) Blum WF, et al. 1997 Plasma Leptin Levels in Healthy Children and Adolescents:Dependence on Body Mass Index，Body Fat Mass,Gender，Pubertal Stage and Testosterone.J Clin Endocrinol metab.82:2904-2910.

37. Blum WF,1997 Leptin: The Voice of the Adipose Tissue.Horm Res.48:2-8.

38. Robinson CJ, Gaines-Das R,Woollacott D, et al.2001 The first international standard for human leptin and the first international standard for mouse leptin: comparison ofcandidate preparations by in vitro bioassays and immunoassays.J Molecular Endocrinol.27 :69-76.

39. Address NIBSC: National Institute for Biological Standards and Controls， Blanche Lane,South Mimms,Potters Bar, Hertfordshire EN6 3QG,Great Britain.

40. Bielohuby M, Matsuura M, Herbach N, Kienzle E, Slawik M, Hoeflich A,Bidlingmaier M. Short Term Exposure to Low-Carbohydrate / High Fat Diets Induces Low Bone Mineral

Density and Reduces Bone Formation in Rats.Journal of Bone and Mineral Research,vol.25,No.2,February 2010, pp 275-284

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